Part:BBa_K4605010:Design

Used to produce indigo in Corynebacterium glutamicum

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3789
  Illegal EcoRI site found at 3940
  Illegal XbaI site found at 25
  Illegal XbaI site found at 9532
  Illegal SpeI site found at 7594
  Illegal SpeI site found at 7973
  Illegal PstI site found at 13
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3789
  Illegal EcoRI site found at 3940
  Illegal SpeI site found at 7594
  Illegal SpeI site found at 7973
  Illegal PstI site found at 13
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3789
  Illegal EcoRI site found at 3940
  Illegal BglII site found at 3932
  Illegal BamHI site found at 31
  Illegal XhoI site found at 3085
  Illegal XhoI site found at 3784
  Illegal XhoI site found at 6063
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3789
  Illegal EcoRI site found at 3940
  Illegal XbaI site found at 25
  Illegal XbaI site found at 9532
  Illegal SpeI site found at 7594
  Illegal SpeI site found at 7973
  Illegal PstI site found at 13
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3789
  Illegal EcoRI site found at 3940
  Illegal XbaI site found at 25
  Illegal XbaI site found at 9532
  Illegal SpeI site found at 7594
  Illegal SpeI site found at 7973
  Illegal PstI site found at 13
  Illegal NgoMIV site found at 331
  Illegal NgoMIV site found at 738
  Illegal NgoMIV site found at 9257
  Illegal NgoMIV site found at 10601
  Illegal AgeI site found at 1996
  Illegal AgeI site found at 2049
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 274
  Illegal BsaI site found at 1585
  Illegal BsaI site found at 2299
  Illegal BsaI site found at 4139
  Illegal BsaI.rc site found at 403
  Illegal BsaI.rc site found at 3589
  Illegal SapI site found at 5029
  Illegal SapI.rc site found at 1837
  

Design Notes

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Source

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